Cloning |
|
Linc-Pint full-length promoter (P0) |
Forward, 5′-AAAGACAATTGGTACCCAAAGTGATG-3′ |
Reverse, 5′-ATCTCTCCCAAGCTTTACCTTCCTATC-3′ |
Linc-Pint P1 |
Forward, 5′-AAAGACAATTGGTACCCAAAGTGATG-3′ |
Reverse, 5′-GTTGACCCCAAGCTTCACAGAGGG-3′ |
Linc-Pint P2 |
Forward, 5′-TACGGCCTGGGTACCTCTCAGCTG-3′ |
Reverse, 5′-ATCTCTCCCAAGCTTTACCTTCCTATC-3′ |
|
ChIP assay |
|
Linc-Pint promoter |
Forward, 5′-CGCCTCCTTCTTGCATGACAC-3′ |
Reverse, 5′-CACAGTGGGGCCAAGGATC-3′ |
|
PCR |
|
Linc-Pint |
Forward, 5′-CGTGGGAGCCCCTTTAAGTT-3′ |
Reverse, 5′-GGGAGGTGGCGTAGTTTCTC-3′ |