Fig 6. Characterization of cancer cell toxicity by different extracts from pomegranate immature and ripe fruits.
Cytotoxicity after treatments with the indicated pomegranate fruit extracts (100 μg/mL for 24 h) was assessed by MTS in H1299 (A) and HCT116 (B) cells. O.D., optical density; H2O2, positive control (100 μM). (C) Caspase-3 activity induced by treatments with BR-B and BG-B (100 μg/ml for 24 h) was quantified with the Ac-DEVD-AMC substrate. Results are fold changes relative to the respective vehicle DMSO. *, p ≤ 0.05; **, p ≤ 0.01 and ***, p ≤ 0.001 vs respective vehicle DMSO. CTR, vehicle control (DMSO).