Fig 4. Overexpressions of chargeable or unchargeable tRNALeu molecules give rise to fully charged tsRNALeu.
(A) Sequence and secondary structure of human tRNALeu and variants. (B) Charging levels of tRNALeu and tsRNALeu in the HeLa cells transfected with plasmids carrying various tRNALeu mutations. ΔAC stands for the tRNALeu truncated variant lacking the anticodon domain. Overexpression of ΔAC variant leads to extra truncated tRNA bands around 70nt size in the northern blot as shown in the middle panel. Let-7a-3p was used as a loading control. (C) Quantification of full-length tRNALeu, charged full-length tRNALeu and tsRNALeu levels when various tRNA mutants were overexpressed. Values are presented as means ± s.d. (n = 4). *, P < 0.05, **, P<0.01, NS, not significant, as determined by Student´s one-tailed t-test.