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. 2021 Jul 27;11:637247. doi: 10.3389/fonc.2021.637247

Figure 1.

Figure 1

Exosomes mediate the transfer of miR-125b into recipient cells. (A) Schematic diagram showing the co-culture experimental procedure. In step 1, exosomes were isolated from Huh7-CM. In step 2, isolated exosomes were loaded with miRNA mimics. In step 3, miRNA-loaded exosomes were purified by TEI. In step 4, purified exosomes were co-cultured with recipient cells. (B) Exosomes were characterized by TEM and NTA. Scale bar, 100 nm. (C) Detection of exosomal markers in cell lysate and exosomes. (D) Upregulation of relative miR-125b expression in Exo-125b compared with that in Exo-NC. (E) Confocal microscopy images of recipient cells treated with Exo-Cy3-125b (Cy3-labeled miR-125b-loaded exosomes) or Exo-NC. Original magnification, ×400 or ×800. Scale bar, 50 µm. Red: Cy3-labeled miR-125b; green: exosome; DAPI: nuclei. (F) Relative miR-125b expression determined by qRT-PCR after recipient cells were treated with Exo-125b or Exo-NC. Data are presented as mean ± SEM. **p < 0.01; ***p < 0.001.