Skip to main content
. 2020 Dec 9;18(9):1354–1363. doi: 10.1080/15476286.2020.1850628

Figure 1.

Figure 1.

Depletion of YTHDF3 results in loss of pluripotency

(A) Western blotting showing knockdown efficiency of YTHDF1 in mouse ESCs. (B) Western blotting showing knockdown efficiency of YTHDF3 in mouse ESCs. (C) Representative image showing morphology (upper panel) and alkaline phosphatase (AP) staining (lower panel) of Ythdf1-knockdown ESC colonies in comparable with control cells (shScram). (D) Representative image showing morphology (upper panel) and AP staining (lower panel) of Ythdf3-knockdown ESC colonies in comparable with control cells (shScram). (E) Detection of pluripotency genes in Ythdf1- and Ythdf3-knockdown ESCs by qRT-PCR. Error bars, mean ± s.e.m.; *P < 0.05, **P < 0.01, unpaired two tailed t-test; n= 4 independent experiments. (F) Quantitative analysis of AP stained colonies formed by Ythdf1- and Ythdf3- knockdown ESCs. Error bars, mean ± s.e.m.; *P < 0.05, unpaired two tailed t-test; n= 3 independent experiments.