Figure 2.

Depletion of YTHDF3 results in the induction of genes involved in formation of three germ layers

(A) GO analysis of DEGs in Ythdf1- (orange) and Ythdf3-knockdown (red) ESCs, compared with control cells. (B) Heat map showing expression of genes responsive for formation of three germ layers, endoderm, ectoderm and mesoderm, in Ythdf1- and Ythdf3-knockdown ESCs. (C) Detection of gene expression for endoderm and mesoderm by qRT-PCR. Error bars, mean ± s.e.m.; **P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 4 independent experiments.