Figure 3.

YTHDF1 and YTHDF3 are downregulated during cardiac differentiation

(A) Detection of YTHDF1 and YTHDF3 protein levels during CMs differentiation by Western blot. (B) Detection of Ythdf1 and Ythdf3 mRNA levels during CMs differentiation by qRT-PCR. (C) Detection of YTHDF1 and YTHDF3 transcript abundance in the presence and absence of leukemia inhibitory factor (LIF) treatment by qRT-PCR. (D) Detection of YTHDF1 and YTHDF3 transcript abundance in ESC and MEF cells by qRT-PCR. Error bars, mean ± s.e.m.; *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 4 independent experiments.