Figure 4.

YTHDF1 and YTHDF3 play divergent roles in ESC-derived cardiac differentiation

(A) Representative morphologic images of Ythdf1- and Ythdf3-knockdown embryoid bodies (EBs) compared with shScram EBs. (B) Beating rate of cardiomyocytes during differentiation in Ythdf-knockdown and shScram cells in the presence of ascorbic acid. (C) Beating rate of cardiomyocytes during differentiation in Ythdf-knockdown and shScram cells in the absence of ascorbic acid. Error bars, mean ± s.e.m.; *P< 0.05, **P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 2 independent experiments, 20 EBs were quantified for each experiment. (D) Representative images of CMs stained by anti-MYH antibody. (E) Detection of pluripotency genes in Ythdf1- and Ythdf3-knockdown cardiomyocytes by qRT-PCR. (F) Detection of mRNA levels of CM marker genes at day 6 in Ythdf-knockdown and shScram cells. Error bars, mean ± s.e.m.; **P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 4 independent experiments.