Figure 6.

Identification of cardiac differentiation phenotypes in cells with depletion of both YTHDF1 and YTHDF3

(A) Western blotting showing double knockdown of Ythdf1 and Ythdf3 (shYthdf1 + 3) in mouse ESCs. (B) Beating rate of cardiomyocytes in double knockdown of Ythdfs cells. Error bars, mean ± s.e.m.; *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 2 independent experiments, 20 EBs were quantified for each experiment. (C) Detection of mRNA levels of CM marker genes in double knockdown of Ythdfs cells. Error bars, mean ± s.e.m.; *P< 0.05**P < 0.01, ***P < 0.001, unpaired two tailed t-test; n= 4 independent experiments.