Figure 5.
Downregulation of dnaA by the sRNA Ec-rnTrpL depends on hfq and rne. Analysis by qRT-PCR of changes in the dnaA mRNA level 3 min after addition of IPTG to strains containing plasmid pSRKTc-Ec-rnTrpL for induced production of the recombinant sRNA lacZ′-Ec-rnTrpL. As a negative control, trpE mRNA was analysed. The dnaA mRNA level after induction was compared to that before induction. Strains with following genetic backgrounds were analysed: (A) the parental strain MG1655 and its mutant MG1655 Δhfq; (B) the parental strain BL322 and its rnc− mutant BL321; (C) the parental strain N3433 and its thermosensitive mutant N3431 (rnets). Cultures were grown in LB medium. The rpoB gene was used as a reference. Shown are means and standard deviations from three independent experiments