Figure 2.

Different patterns of mRNA localization revealed by FISH and smFISH

A – FISH on Drosophila embryo (mRNA green/nuclei red). The gene names are indicated on the bottom left and localization patterns above the photos. From left to right: bicoid mRNA displays anterior localization in the embryo; anillin mRNA has perinuclear localization; CG1962 mRNA localizes to microtubules networks and centrosomes during cell division. Reprinted from Lécuyer E, Yoshida H, Parthasarathy N, Alm C, Babak T, Cerovina T, Hughes TR, Tomancak P, Krause HM. Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function. Cell 2007; 131:174–87. Pages 178, 180, 181. Copyright 2020, with permission from Elsevier. B – smFISH on Hela cells. The genes names are indicated on the bottom left and localization patterns are above the photos. Top – from left to right: KIF5B is localized to the cell protrusions; FLNA is localized to the cell edge; BUB1 – cytoplasmic foci. Orange arrows indicate mentioned locations. Bottom – ASPM mRNA is localized to nuclear envelope and cytoplasmic foci in interphase and to spindle pole during cell division. Left – smFISH, right – nuclei stained with DAPI. Orange arrow indicates interphase cell; pink arrow – mitotic cell. Reprinted from Chouaib R, Safieddine A, Pichon X, Imbert A, Kwon OS, Samacoits A, Traboulsi A-M, Robert M-C, Tsanov N, Coleno E, et al. A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting. Dev Cell 2020; 54:773–791.e5. Pages 775, 776. Copyright 2020, with permission from Elsevier.