Figure 3.

Vac8 vacuolar membrane localization is required for correct Atg13 localization. (A) Atg13-RFP association with the vacuole was determined by fluorescence microscopy after 1 h of nitrogen starvation in strains expressing Vac8-AID-MYC and either Vac8-GFP WT, Vac8-GFP Cyt or Vac8-GFP ER. Strains were treated for 30 min with either IAA to induce Vac8-AID-MYC degradation, or control vehicle DMSO. The vacuolar lumen was stained using CMAC. (B) Quantification of panel (A). The percentage of cells in which Atg13-RFP puncta associated with the vacuole is presented. ANOVA, ***P < 0.001. ns, no significance