Figure 4.

Vac8 vacuolar membrane localization is required for correct Atg9 anterograde traffic. (A) Atg9-RFP association with the vacuole was monitored using fluorescence microscopy after 1 h of nitrogen starvation in atg1∆ strains expressing Vac8-AID-MYC and either Vac8-GFP WT, Vac8-GFP Cyt or Vac8-GFP ER. Strains were treated for 30 min with either IAA to induce Vac8-AID-MYC degradation, or vehicle DMSO as a control. The vacuolar lumen was stained using CMAC. (B) Quantification of panel (A). The percentage of cells in which Atg9-RFP puncta associated with the vacuole is presented. ANOVA, *P < 0.05; ***P < 0.001. ns, no significance