Table 1.
Yeast strains used in this study
| Strain | Genotype | Reference |
|---|---|---|
| DGY056 | WLY176 vac8∆::HIS3 | This study |
| DGY057 | WLY176 atg13∆::HIS3 | This study |
| DGY058 | WLY176 GFP-ATG8::URA3 | This study |
| DGY059 | DGY058 vac8∆::HIS3 | This study |
| DGY060 | SEY6210 pho8::pho8∆60 pNHK53::URA3 VAC8-AID-MYC::HIS3 | This study |
| DGY061 | SEY6210 GFP-ATG8::LEU2 pNHK53::URA3 VAC8-AID-MYC::HIS3 | This study |
| DGY062 | WLY176 VAC8-GFP::HIS3 | This study |
| DGY063 | WLY176 VAC8G2A-GFP::HIS3 | This study |
| DGY064 | WLY176 VAC8C4,5,7A-GFP::HIS3 | This study |
| DGY065 | SEY6210 pNHK53::URA3 VAC8-AID-MYC::HIS3 VAC8-GFP::LEU2 | This study |
| DGY066 | SEY6210 pNHK53::URA3 VAC8-AID-MYC::HIS3 VAC8G2A,C4A,C5A,C7A-GFP::LEU2 | This study |
| DGY067 | SEY6210 pNHK53::URA3 VAC8-AID-MYC::HIS3 SEC66-VAC8-GFP::LEU2 | This study |
| DGY068 | DGY065 RFP-APE1::TRP1 | This study |
| DGY069 | DGY066 RFP-APE1::TRP1 | This study |
| DGY070 | DGY067 RFP-APE1::TRP1 | This study |
| DGY071 | DGY056 VAC8-GFP::LEU2 | This study |
| DGY072 | DGY056 VAC8G2A,C4A,C5A,C7A-GFP::LEU2 | This study |
| DGY073 | DGY056 SEC66-VAC8-GFP::LEU2 | This study |
| DGY074 | DGY065 ATG13-RFP::TRP1 | This study |
| DGY075 | DGY066 ATG13-RFP::TRP1 | This study |
| DGY076 | DGY067 ATG13-RFP::TRP1 | This study |
| DGY077 | DGY065 ATG1-RFP::TRP1 | This study |
| DGY078 | DGY066 ATG1-RFP::TRP1 | This study |
| DGY079 | DGY067 ATG1-RFP::TRP1 | This study |
| DGY080 | DGY065 ATG9-RFP::TRP1 atg1∆::NAT | This study |
| DGY081 | DGY066 ATG9-RFP::TRP1 atg1∆::NAT | This study |
| DGY082 | DGY067 ATG9-RFP::TRP1 atg1∆::NAT | This study |
| DGY083 | WLY176 VAC8-RFP::LEU2 ATG13-GFP::TRP1 | This study |
| DGY084 | WLY176 PEX14-VAC8-RFP::LEU2 ATG13-GFP::TRP1 | This study |
| DGY085 | WLY176 VAC8-RFP::LEU2 PEX14-GFP::TRP1 | This study |
| DGY086 | WLY176 PEX14-VAC8-RFP::LEU2 PEX14-GFP::TRP1 | This study |
| DGY087 | DGY085 atg36∆::NAT | This study |
| DGY088 | DGY086 atg36∆::NAT | This study |
| DGY089 | WLY176 ATG13-GFP::TRP1 | This study |
| DGY090 | WLY176 ATG13[1-659]-GFP::TRP1 | This study |
| DGY091 | DGY090 vac8∆::HIS3 | This study |
| vac8-2 |
MATα leu2-112, 2–3 ura3-52 trp1-∆901 lys2-801 suc2-∆9 vac8∆::HIS3 Vac8G2 S |
[11] |
| vac8-3 |
MATα leu2-112, 2–3 ura3-52 trp1-∆901 lys2-801 suc2-∆9 vac8∆::HIS3 Vac8C4 G,C5 T,C7 S |
[11] |
| [20] | ||
| vac8-15ts |
MATα leu2-112, 2–3 ura3-52 trp1-∆901 lys2-801 suc2-∆9 vac8∆::HIS3 vac8-15ts |
|
| WLY176 | SEY6210 pho13∆ pho8::pho8∆60 | [17] |