Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-FLAG (Rabbit) | Sigma-Aldrich | Cat# F7425, RRID:AB_439687 | WB (1:2000) |
| Antibody | anti-Actin clone C4 (Mouse monoclonal) | MP Biomedicals | Cat# 08691002, RRID:AB_2335304 | WB (1:2000) |
| Antibody | Anti-FLAG M2 Magnetic Beads | Sigma-Aldrich | Cat# M8823, RRID:AB_2637089 | IP |
| Recombinant protein reagent | Cas9-NLS (purified protein) | UC Berkely QB3 | ||
| Genetic reagent (C. elegans) |
+ | CGC | RRID:CGC_N2 | |
| Genetic reagent (C. elegans) |
acr-2(n2420) X | Jospin et al., 2009 | MT6241 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II | This work | CZ22197 | Figure 1F |
| Genetic reagent (C. elegans) |
eif-3.G(ju1840) II | This work | CZ28494 | Figure 1C |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ21759 | Figure 1C |
| Genetic reagent (C. elegans) |
eif-3.G(ju1840) II; acr-2(n2420) X | This work | CZ28495 | Figure 1C |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) / mnC1 II | This work | CZ22974 | Figure 1F |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7015 | This work | CZ22976 | Figure 1C |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7016 | This work | CZ22977 | Figure 1C |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) I;acr-2(n2420) X; juEx7045 | This work | CZ23125 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7046 | This work | CZ23126 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7019 | This work | CZ22980 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7020 | This work | CZ22981 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7439 | This work | CZ23791 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7440 | This work | CZ23880 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7021 | This work | CZ22982 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx7022 | This work | CZ22983 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx8062 | This work | CZ27881 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; juEx8063 | This work | CZ27882 | Figure 1E |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) /mnC1 II; acr-2(n2420) X | This work | CZ23310 | Figure 1F |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) / eif-3.G(ju1327) II | This work | CZ25714 | Figure 1F |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) / eif-3.G(ju1327) II; acr-2(n2420) X | This work | CZ26828 | Figure 1F |
| Genetic reagent (C. elegans) |
juSi320 IV | This work | CZ24063 | Figure 2B; Figure 1—figure supplement 1B |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) /mnC1 II; juSi320 IV | This work | CZ24079 | Figure 2A |
| Genetic reagent (C. elegans) |
juSi320 IV; acr-2(n2420) X | This work | CZ24729 | Figure 2A |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; juSi320 IV; acr-2(n2420) X | This work | CZ28107 | Figure 2A |
| Genetic reagent (C. elegans) |
juSi331 IV | This work | CZ24651 | Figure 2B; Figure 1—figure supplement 1B |
| Genetic reagent (C. elegans) |
juSi331 IV; acr-2(n2420) X | This work | CZ24652 | Figure 2A |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) / mnC1 II; juSi331 IV; acr-2(n2420) X | This work | CZ28497 | Figure 2A |
| Genetic reagent (C. elegans) |
juIs14 IV | Wang et al., 2017 | CZ631 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; juIs14 IV | This work | CZ24161 | Figure 1—figure supplement 2 |
| Genetic reagent (C. elegans) |
juIs14 IV; acr-2(n2420) X | McCulloch et al., 2020 | CZ5808 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; juIs14 IV; acr-2(n2420) X | This work | CZ8905 | Figure 1—figure supplement 2A |
| Genetic reagent (C. elegans) |
nuIs94 | Hallam et al., 2000 | KP2229 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; nuIs94 | This work | CZ24021 | Figure 1—figure supplement 2B |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; nuIs94 | This work | CZ5815 | Figure 1—figure supplement 2B |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420)X; nuIs94 | This work | CZ24021 | Figure 1—figure supplement 2B |
| Genetic reagent (C. elegans) |
eif-3.E(ok2607) I / hT2 I,III; acr-2(n2420) X | This work | CZ27434 | Figure 1—figure supplement 3A |
| Genetic reagent (C. elegans) |
eif-3.E(ok2607) I / hT2 I, III; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27433 | Figure 1—figure supplement 3A |
| Genetic reagent (C. elegans) |
eif-3.H(ok1353) I / hT2 I, III; acr-2(n2420) X | This work | CZ27435 | Figure 1—figure supplement 3A |
| Genetic reagent (C. elegans) |
eif-3.H(ok1353) I / hT2 I, III; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27436 | Figure 1—figure supplement 3A |
| Genetic reagent (C. elegans) |
oxSi39 IV | Qi et al., 2013 | CZ12338 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; oxSi39 IV | This work | CZ23854 | Figure 1—figure supplement 3B |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7056 | This work | CZ23203 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7057 | This work | CZ23204 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8100 | This work | CZ28152 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8101 | This work | CZ28153 | Figure 3 |
| Genetic reagent (C. elegans) |
juEx7113 | This work | CZ26777 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7114 | This work | CZ23304 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7115 | This work | CZ23305 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8095 | This work | CZ28066 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8096 | This work | CZ28067 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8087 | This work | CZ28057 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8088 | This work | CZ28058 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8089 | This work | CZ28064 | Figure 3 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx8090 | This work | CZ28065 | Figure 3 |
| Genetic reagent (C. elegans) |
unc-119(tm4063) III; wgIs433 | Sarov et al., 2006 | OP433 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; unc-119(tm4063)III; wgIs433 | This work | CZ28145 | Figure 6C |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; unc-119(tm4063) III; wgIs433 | This work | CZ27913 | Figure 6C |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; unc-119(tm4063) III; acr-2(n2420) X; wgIs433 | This work | CZ27914 | Figure 6C |
| Genetic reagent (C. elegans) |
sqIs17 | Dittman and Kaplan, 2006 | MAH240 | |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; sqIs17 | This work | CZ28334 | Figure 6—figure supplement 1 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; sqIs17 | This work | CZ28212 | Figure 6—figure supplement 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; sqIs17 | This work | CZ28218 | Figure 6—figure supplement 1 |
| Genetic reagent (C. elegans) |
unc-13(s69) I; wgIs433 | This work | CZ28491 | Figure 6C |
| Genetic reagent (C. elegans) |
unc-13(s69) I; acr-2(n2420) X; wgIs433 | This work | CZ28492 | Figure 6C |
| Genetic reagent (C. elegans) |
unc-13(s69) I; eif-3.G(ju807); acr-2(n2420) X; wgIs433 | This work | CZ28493 | Figure 6C |
| Genetic reagent (C. elegans) |
juSi260 ncs-2(tm1943) I | Zhou et al., 2017 | CZ22459 | |
| Genetic reagent (C. elegans) |
juSi260 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ23225 | Figure 7B |
| Genetic reagent (C. elegans) |
juSi260 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ22345 | Figure 7B |
| Genetic reagent (C. elegans) |
juSi260 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28110 | Figure 7B |
| Genetic reagent (C. elegans) |
juSi391 ncs-2(tm1943) I | This work | CZ28213 | Figure 7C |
| Genetic reagent (C. elegans) |
juSi391 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ28340 | Figure 7C |
| Genetic reagent (C. elegans) |
juSi391 ncs-2(tm1943) I;acr-2(n2420) X | This work | CZ28252 | Figure 7C |
| Genetic reagent (C. elegans) |
juSi391 ncs-2(tm1943) I;eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28253 | Figure 7C |
| Genetic reagent (C. elegans) |
juSi392 ncs-2(tm1943) I | This work | CZ28277 | Figure 7—figure supplement 1B |
| Genetic reagent (C. elegans) |
juSi392 ncs-2(tm1943) I;eif-3.G(ju807) II | This work | CZ28312 | Figure 7—figure supplement 1B |
| Genetic reagent (C. elegans) |
juSi392 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ28291 | Figure 7—figure supplement 1B |
| Genetic reagent (C. elegans) |
juSi392 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28292 | Figure 7—figure supplement 1B |
| Genetic reagent (C. elegans) |
juSi393 ncs-2(tm1943) I | This work | CZ28278 | Figure 7—figure supplement 1C |
| Genetic reagent (C. elegans) |
juSi393 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ28311 | Figure 7—figure supplement 1C |
| Genetic reagent (C. elegans) |
juSi393 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ28293 | Figure 7—figure supplement 1C |
| Genetic reagent (C. elegans) |
juSi393 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28294 | Figure 7—figure supplement 1C |
| Genetic reagent (C. elegans) |
juEx2045 | --- | CZ9635 | |
| Genetic reagent (C. elegans) |
hlh-30(tm1978) IV | CGC | CZ23321 | |
| Genetic reagent (C. elegans) |
hlh-30(tm1978) IV; acr-2(n2420) X | This work | CZ28174 | Related to Figure 6C |
| Genetic reagent (C. elegans) | eif-3.G(ju807) II; hlh-30(tm1978) IV; acr-2(n2420) X | This work | CZ28175 | Related to Figure 6C |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) II /mnC1; juSi363 IV; acr-2(n2420) X | This work | CZ26759 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
eif-3.G(ju1327) II / mnC1 II; juSi366 IV; acr-2(n2420) X | This work | CZ26760 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
juSi364 IV; acr-2(n2420) X | This work | CZ26494 | Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) | eif-3.G(ju807)II juSi364 IV; acr-2(n2420) X | This work | CZ26243 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
juSi365 IV | This work | CZ26588 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; juSi365 IV; acr-2(n2420) X | This work | CZ26565 | Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
juSi365 IV; acr-2(n2420) X | This work | CZ26566 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
juSi368 IV | This work | CZ26656 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
juSi368 IV; acr-2(n2420) X | This work | CZ26623 | Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; juSi368 IV; acr-2(n2420) X | This work | CZ26480 | Related to Figure 4—figure supplement 1A |
| Genetic reagent (C. elegans) |
wgIs506 | Sarov et al., 2006 | OP506 | Supplementary file 1 |
| Genetic reagent (C. elegans) | acr-2(n2420) X; wgIs506 | This work | CZ27926 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; wgIs506 | This work | CZ27927 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
dhc-1::GFP(it45) I | Lapierre et al., 2013 | OD2955 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
dhc-1::GFP(it45) I; acr-2(n2420) X | This work | CZ27858 | Supplementary file 1 |
| Genetic reagent (C. elegans) | dhc-1::GFP(it45) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27859 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
wgIs432 | Sarov et al., 2006 | OP432 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; wgIs432 | This work | CZ27915 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; wgIs432 | This work | CZ28021 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
wgIs638 | Sarov et al., 2006 | OP638 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
unc-119(tm4063) III;acr-2(n2420) X; wgIs638 | This work | CZ28108 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; wgIs638 | This work | CZ27916 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
let-607(tm1423) I; unc-119(ed3) III; vrIs121 | Sarov et al., 2006 | YL651 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; let-607(tm1423) I; unc-119(ed3) III; vrIs121 | This work | CZ28143 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; let-607(tm1423) I;unc-119(ed3) III; vrIs121 | This work | CZ28119 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
eif-3.G(ju807) II; acr-2(n2420) X; let-607(tm1423) I; unc-119(ed3) III; vrIs121 | This work | CZ28111 | Supplementary file 1 |
| Genetic reagent (C. elegans) |
juIs172 | CGC | EE86 | |
| Genetic reagent (C. elegans) |
egl-30(md186) I;dpy-20(e1282ts) IV; syIs105 | CGC | PS4263 | |
| Genetic reagent (C. elegans) |
juEx7964 | McCulloch et al., 2020 | CZ27420 | |
| Genetic reagent (C. elegans) |
acr-2(n2420) X; juEx7964 | McCulloch et al., 2020 | CZ27217 | |
| Genetic reagent (C. elegans) |
eif-3.G(C130Y) II; acr-2(n2420) X; juEx7964 | This work | CZ28109 | Supplementary file 1 |
| Recombinant DNA reagent (plasmid) | pCZGY2729 | Andrusiak et al., 2019 | RRID:Addgene_135096 | Site-specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV |
| Recombinant DNA reagent (plasmid) | pCZGY2750 | Andrusiak et al., 2019 | RRID:Addgene_135094 | Expresses Cas9 and sgRNA for editing of C. elegans ChrIV |
| Recombinant DNA reagent (plasmid) | pCZGY2727 | This work | Site-specific insertion using CRISPR/Cas9 editing of C. elegans ChrI | |
| Recombinant DNA reagent (plasmid) | pCZGY2748 | This work | Expresses Cas9 and sgRNA for editing of C. elegans ChrI |