Figure 6. Comparison of spontaneous and stimulus-driven activity in the mouse A1.

(A) A sample trial sequence in the experiment. Stimulus-driven (stim) trials were recorded with randomly interleaved spontaneous (spon) trials of the same duration. (B) Estimated noise and signal correlation matrices under spontaneous (top) and stimulus-driven (bottom) conditions. Rows from left to right: proposed method, Pearson correlations from two-photon data, two-stage Pearson and two-stage GPFA estimates. (C) Location of the selected neurons with highest activity in the field of view. (D) Stimulus onsets (orange), observations (black), estimated calcium concentrations (purple) and putative spikes (green) for the first trial from two pairs of neurons with high signal correlation (top) and high noise correlation (bottom), as identified by the proposed estimates.

Figure 6—figure supplement 1. Histograms of the similarity/dissimilarity metrics under the shuffling procedure.

Null distributions of (A) the similarities between ${\mathbf{𝐍}}_{\mathrm{𝗌𝗉𝗈𝗇}}$ and ${\mathbf{𝐍}}_{\mathrm{𝗌𝗍𝗂𝗆}}$ (top: $T_s({\widehat{\mathbf{𝐍}}}_{\mathrm{𝗌𝗉𝗈𝗇}},{\widehat{\mathbf{𝐍}}}_{\mathrm{𝗌𝗍𝗂𝗆}})$) and (B) the dissimilarities between ${\widehat{\mathbf{𝐒}}}_{\mathrm{𝗌𝗍𝗂𝗆}}$ and ${\widehat{\mathbf{𝐍}}}_{\mathrm{𝗌𝗍𝗂𝗆}}$ (bottom: $T_d({\widehat{\mathbf{𝐒}}}_{\mathrm{𝗌𝗍𝗂𝗆}},{\widehat{\mathbf{𝐍}}}_{\mathrm{𝗌𝗍𝗂𝗆}})$), obtained by the shuffling procedure applied to the results of real data study 2 in Figure 6. The observed test statistic in each case is indicated by a dashed vertical line. Rows from left to right: proposed method, Pearson correlations from two-photon data, two-stage Pearson correlations and two-stage GPFA estimates. These results show that the only statistically significant outcomes (with $p\le 0.05$) are the similarities and dissimilarities obtained by our proposed method.