Figure 4.

The inhibitors of cholesterol biosynthesis impair rotavirus replication. With rotavirus SA11 strain (MOI 0.7) infection and subsequently treated with different concentrations of 6-fluoromevalonate for 48 hours. The intra- and extracellular rotavirus RNA levels were measured by qRT-PCR (n = 6) (left) and the expressions of rotavirus VP4 protein were tested by western blot (right) in Caco2 cells (a), and in MA104 cells (b). With ZA-A treatments, the intra- and extracellular rotavirus RNA levels (n = 9) (left) and the expressions of rotavirus VP4 protein (right) in Caco2 cells (c), and in MA104 cells (d). with 1.25 µM U18666A treatment, the intra- and extracellular rotavirus RNA levels (n = 6) (left) and the expressions of rotavirus VP4 protein (right) in Caco2 cells (e), and in MA104 cells (f). In HSI organoids, the inner rotavirus RNA level with 200 µM 6-fluoromevalonate (n = 5) (g), 50 µM ZA-A (n = 5) (h) and 1.25 µM U18666A (n = 4) (i); and the expressions of rotavirus VP4 protein with 200 µM 6-fluoromevalonate or 50 µM ZA-A treatment (left) (j), with 1.25 µM U18666A treatment (right) (k). All data presented as mean ± SEM, *p < .05, **p < .01, ***p < .001