Figure 6.

Enhancement of cholesterol production provokes rotavirus replication. With rotavirus SA11 strain (MOI 0.7) infection and subsequently treated with different concentrations of R-MA for 48 hours and subsequently the intra- and extracellular rotavirus RNA levels were measured by qRT-PCR (n = 5) (left) and the expressions of rotavirus VP4 protein were tested by western blot (right) in Caco2 cells (a), and in MA104 cells (b). With different concentrations of cholesterol treatment, the intra- and extracellular rotavirus levels (n = 5) (left) and the expressions of rotavirus VP4 protein (right) in Caco2 cells (c), and in MA104 cells (d). In HSI organoids, the inner rotavirus RNA level (n = 3) (left) and the expressions of rotavirus VP4 protein (right) with R-MA treatment (e), with cholesterol treatment (f). (g) The titers of infectious rotavirus particles were measured by TCID₅₀ assay upon R-MA (n = 6) (left) or cholesterol (n = 6) (right) treatment respectively in MA104 cells. (h) The time-course experiment of 150 µM cholesterol treatment on the intracellular rotavirus replication. All data presented as mean ± SEM, *p < .05, **p < .01, ***p < .001