Table 1.
Primers used for hippocampus and hypothalamus qPCR in male and female CF1 mice offspring
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Efficiency |
|---|---|---|---|
| Avp | ATCTGCTGCAGCGACGAGAG | TGTACCAGCCTTAGCAGCAG | 90.3 |
| B2m1 | CCTTCAGCAAGGACTGGTCT | TTGATCACATGTCTCGATCCCAG | 100.3 |
| Bdnf | CGGAGAGCAGAGTCCATTCAG | CTCACCTGGTGGAACTTCTTTG | 90.7 |
| Cnrip1 | AACACTGCAGGTCGAGAACATT | TTGCCACACTGTCTCGAAGG | 91.3 |
| DAT | CTGGAAGATCTGCCCTGTCC | CAGCACACCACGCTCAAAAT | 103.9 |
| Dnmt3a | GATGAGCCTGAGTATGAGGATGG | CAAGACACAATTCGGCCTGG | 103.6 |
| Dnmt3b | CTGTCCGAACCCGACATAGC | CCGGAAACTCCACAGGGTA | 96.4 |
| Drd1 | TTGAGTCCAGGGGTTTTGGG | GGGCCTCTTCCTGGTCAATC | 99.0 |
| Drd2 | TGAACAGGCGGAGAATGGATG | GTTTTGCCATTGGGCATGGT | 90.0 |
| Esr1 | CTTGAACCAGCAGGGTGGC | AGGCTTTGGTGTGAAGGGTC | 100.2 |
| Esr2 | ACGAAGAGTGCTGTCCCAAG | GCCAAGGGGTACATACTGGAG | 112.0 |
| Gnrh | CAACTGTGCTCACCAGCGG | TGAGGATCATGTCCACTCTGTTT | 110.0 |
| Htr2a | CACCGACATGCCTCTCCATT | GGCCACCGGTACCCATACAG | 90.9 |
| Htr7 | GGGACCTGAGGACCACCTAT | CAGTGGTCACAGTTTTGTAGCA | 98.0 |
| Kiss1 | AGACTGTAGACCTGCCCCTT | GACTGCTGGCCTGTGGAT | 106.7 |
| Lepr | CAGAATGACGCAGGGCTGTA | GCTCAAATGTTTCAGGCTTTTGGA | 92.7 |
| Oprd1 | CCGTGGCCTCCGTTTTCC | CAATTTGGTGTACCGGACGA | 95.0 |
| Oprk1 | AGTGCCACCTTCTCGCTTT | GGTCTTCATCTTCGTGTATCGGA | 99.3 |
| Oprl1 | TCTTCGGAGCAGGAGCTAGG | GCCACTCAGTACAGTTCCTCC | 105.4 |
| Oprm1 | CATGGCCCTCTATTCTATCGTGT | CCACGTTCCCATCAGGTAGTT | 99.4 |
| Oxtr | GTCTGGTCAAATACTTGCAGGT | CGCGCAGCGAGAAAATGTG | 97.6 |
| Rpl7 | AGCTGGCCTTTGTCATCAGAA | GACGAAGGAGCTGCAGAACCT | 96.1 |
| Slc6a4 | CTCCGCAGTTCCCAGTACAAG | CACGGCATAGCCAATGACAGA | 108.5 |