Table 1.
Primer table
| Gene name (encodes for) | Accession number | Primer location (nt) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| Kiss1 (Kiss1)a | NM_178260 | 64–80 | 120 | 57 |
| 167–183 | ||||
| Slc32a1 (vGAT)a | NM_009508 | 813–834 | 137 | 59 |
| 928–949 | ||||
| Slc17a6 (vGluT2)a,b | NM_080853 | 1677–1694 | 136 | 57 |
| 1795–1812 | ||||
| Pdyn (Dyn)b | NM_018863 | 210–228 | 154 | 58 |
| 345–363 | ||||
| Cart (CART)b | NM_013732 | 288–305 | 157 | 59 |
| 427–444 | ||||
| Lepr (LeptRec)b | NM_146146 | 3185–3205 | 112 | 62 |
| 3276–3296 |
a
primers used for identifying dispersed single neurons.
b
primers used for identifying single neurons after electrophysiological recording.