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. 2021 Aug 11;116(6):1631–1640. doi: 10.1016/j.fertnstert.2021.08.009

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©2021 American Society for Reproductive Medicine, Published by Elsevier Inc.

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Supplemental Figure 4 — Effects of hCG on NR3C1 expression and cortisol production in hGLCs. Granulosa/lutein cells obtained from IVF patients were cultured for 6 d and then treated with or without hCG (1 IU/) for 36 hours. (A) NR3C1 was detected by Western blots using whole-cell extracts. The membrane was reprobed with a monoclonal antibody against ACTB to assess protein loading in each lane. (B) Cortisol levels in culture media were measured by a cortisol ELISA kit (R&D systems) after performing extraction procedures described by Yong et. al (PMID: 11134135) (n = 4 individual patient samples; ∗, p ≤ 0.05).