Figure 2.

Changes in mice. Male mice were exposed to air at ambient pressure (control) or for 2 h to 790 kPa air, decompressed and euthanized 2 h later (Deco). Where indicated air-exposed control mice were injected intravenously with 27 mg/kg rhu-pGSN (Control + Rhu-pGSN) and euthanized 4 h later. Other mice were injected with rhu-pGSN prior to pressurization (Rhu-pGSN + Deco) or immediately after decompression (Deco + Rhu-pGSN), and others injected intravenously with the carrier buffer used to suspend rhu-pGSN (Vehicle + Deco), and these groups euthanized 2 h after decompression. The concentrations of mouse pGSN and IL-1β were measured in plasma samples by mouse-specific ELISAs and blood-borne MPs were quantified as described in methods. Data are expressed as means ± SE, the (n) for each sample is shown, *indicates significantly different from control, P < 0.05, ANOVA. IL-1β, interleukin-1β; MP, microparticles; rhu-pGSN, recombinant human-plasma gelsolin; pGSN, plasma gelsolin.