Figure 3.

Biotinylation of MPs proteins. MPs from control and decompressed male mice were isolated, incubated with 200 mg/mL rhu-pGSN (shown as +pGSN) or just PBS, and then biotinylated as described in methods. MPs were then lysed in SDS buffer and protein from 45,500 MPs loaded into each lane for SDS-PAGE. Western blots probed for biotin and for β-actin are shown. Probing for IL-1β did not demonstrate bands (not shown). Molecular weight standards (in kDa) are shown at left. IL-1β, interleukin-1β; MP, microparticles; rhu-pGSN, recombinant human-plasma gelsolin; pGSN, plasma gelsolin.