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. 2021 Mar 25;130(5):1604–1613. doi: 10.1152/japplphysiol.01062.2020

Table 3.

MPs subtypes in mice

%Ly6G %CD14 %CD31 %CD41 %ECs %Phall.
Control (19) 0.1 ± 0.1 44.3 ± 4.1 47.8 ± 5.7 29.1 ± 3.7 0.6 ± 0.1 2.3 ± 0.6
Control + pGSN (1) 1.0 ± 0.5 45.9 ± 5.1 36.1 ± 4.9 23.6 ± 4.4 0.8 ± 0.2 3.2 ± 0.7
Deco (1) 11.1 ± 1.9* 51.2 ± 3.5 36.8 ± 3.4 18.0 ± 3.3 2.3 ± 0.3* 29.4 ± 3.3*
Vehicle + Deco (1) 14.3 ± 2.5* 47.1 ± 3.3 49.4 ± 4.5 19.7 ± 3.4 1.7 ± 0.2* 24.5 ± 2.5*
pGSN + Deco (1) 3.9 ± 0.9* 50.6 ± 3.2 38.9 ± 3.6 21.1 ± 3.5 0.4 ± 0.1 4.5 ± 1.0
Deco + pGSN (1) 5.3 ± 0.8* 54.9 ± 6.8 40.2 ± 8.1 30.0 ± 5.7 0.5 ± 0.1 6.2 ± 2.0

Blood-borne MPs were quantified in male mice manipulated as described in the caption for Fig. 2 Flow cytometric measurements were made to quantify the number of all 0.3 to 1 µm diameter Annexin V-positive particles (data in Fig. 2) as well as the fraction of those expressing proteins specific to certain cells [Ly6G (mature neutrophils), CD14 (all leukocytes), CD31 (platelets and endothelium), CD41 (platelets), CD31+/ CD41-dim (endothelium, labeled ECs)] and also those that bound phalloidin (Phall). Data are expressed as means ± SE, n is shown for each sample; asterisk (*) and boldface indicates significantly different from control, P < 0.05, ANOVA. MP, microparticles; pGSN, plasma gelsolin.