Table 3.
%Ly6G | %CD14 | %CD31 | %CD41 | %ECs | %Phall. | |
---|---|---|---|---|---|---|
Control (19) | 0.1 ± 0.1 | 44.3 ± 4.1 | 47.8 ± 5.7 | 29.1 ± 3.7 | 0.6 ± 0.1 | 2.3 ± 0.6 |
Control + pGSN (1) | 1.0 ± 0.5 | 45.9 ± 5.1 | 36.1 ± 4.9 | 23.6 ± 4.4 | 0.8 ± 0.2 | 3.2 ± 0.7 |
Deco (1) | 11.1 ± 1.9* | 51.2 ± 3.5 | 36.8 ± 3.4 | 18.0 ± 3.3 | 2.3 ± 0.3* | 29.4 ± 3.3* |
Vehicle + Deco (1) | 14.3 ± 2.5* | 47.1 ± 3.3 | 49.4 ± 4.5 | 19.7 ± 3.4 | 1.7 ± 0.2* | 24.5 ± 2.5* |
pGSN + Deco (1) | 3.9 ± 0.9* | 50.6 ± 3.2 | 38.9 ± 3.6 | 21.1 ± 3.5 | 0.4 ± 0.1 | 4.5 ± 1.0 |
Deco + pGSN (1) | 5.3 ± 0.8* | 54.9 ± 6.8 | 40.2 ± 8.1 | 30.0 ± 5.7 | 0.5 ± 0.1 | 6.2 ± 2.0 |
Blood-borne MPs were quantified in male mice manipulated as described in the caption for Fig. 2 Flow cytometric measurements were made to quantify the number of all 0.3 to 1 µm diameter Annexin V-positive particles (data in Fig. 2) as well as the fraction of those expressing proteins specific to certain cells [Ly6G (mature neutrophils), CD14 (all leukocytes), CD31 (platelets and endothelium), CD41 (platelets), CD31+/ CD41-dim (endothelium, labeled ECs)] and also those that bound phalloidin (Phall). Data are expressed as means ± SE, n is shown for each sample; asterisk (*) and boldface indicates significantly different from control, P < 0.05, ANOVA. MP, microparticles; pGSN, plasma gelsolin.