Fig. 2.

Kisspeptin (KP, red), type 1 cannabinoid receptor (CB1, blue), and vesicular GABA (VGAT, green) or glutamate transporter (VGLUT2, green) transcripts detected by the RNAscope in situ hybridization technique in the medial basal hypothalamus of OVX + Oil mice. DAPI counterstaining (grey–white spots) demonstrates the cell nuclei and supports the area and cell-specific identification of the different transcripts (A and C). The KP mRNA signal is present in the arcuate nucleus, where a low level of CB1 transcript can also be detected (B and D). In contrast, the VMH shows a much stronger CB1 mRNA signal in co-distribution almost exclusively with the VGULT2 mRNA signal (B and D). Scale bar 100 µm