Table 1.

Primary and secondary antibodies used in concurrent detection of three different tissue antigens in sections of ovariectomized 17β-estradiol benzoate-treated wild-type and Kiss1-CRE-GFP mice

Detection of	GnRH-positive cells	KP-positive cells	CB1-positive cells
In sections of wild-type CD1 mice
PABs	Guinea pig anti-GnRH (#1018, gift from Dr. Erik Hrabovszky, 1:50,000)	Sheep anti-KP (#053, gift from Dr. Alain Caraty, 1:1000)	Rabbit anti-CB1 (ImmunoGenes-ABS Zrt, 1:1000)
SABs	FITC-donkey anti-guinea pig IgG (H + L) (#706-095-148, Jackson Immuno Research Laboratories, 1:500)	CY3-donkey anti-sheep IgG (Fab) 2 (#713-165-147, Jackson Immuno Research Laboratories, 1:3000)	CY5-donkey anti-rabbit IgG (H + L), (#711-175-152, Jackson Immuno Research Laboratories, 1:2000)
In sections of Kiss1-Cre-GFP mice injected with AAV-EF1A-DIO-hChR2 (H134R)-EYFP
PABs	Guinea pig anti-GnRH (#1018, gift from Dr. Erik Hrabovszky, 1:50,000)	Rabbit anti-GFP, (AB10145, Millipore, 1:2000)	Goat anti-CB1 (gift from Dr. Masahiko Watanabe, 1:600)
SABs	CY5- conjugated donkey anti-guinea pig IgG (#706-175-148, Jackson Immuno Research Laboratories, 1:2000)	FITC-conjugated donkey anti-rabbit IgG (#711-095-152, Jackson Immuno Research Laboratories, 1:1000)	CY3-conjugated donkey anti-goat IgG (#706-165-147, Jackson Immuno Research Laboratories, 1:2000)