Fig. 2.
Effects of fluoxetine treatment cessation and ethanol drinking reinstatement on the number of IBA-1 + microglial cells and IBA-1 immunoreactivity in the prelimbic cortex (A, B), striatum (D, E) and basolateral amygdala (G, H). The histograms represent the mean + SEM of cells per area (mm2) and arbitrary units of immunoreactivity (n = 6 rats per experimental group). Tukey’s test: **/***p < 0.01/0.001 vs. saccharine-vehicle group; $$$p < 0.001 vs. saccharine-fluoxetine group. Representative microphotographs showing magnification views of the immunostaining in the prelimbic cortex (C), striatum (F) and basolateral amygdala (I). Scale bars are included in representative images