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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cancer Immunol Res. 2021 Mar 24;9(6):651–664. doi: 10.1158/2326-6066.CIR-20-0445

Figure 4: Blocking β-AR signaling in stressed mice increases expression of anti-tumor cytokines and cytotoxic proteins in TILs.

Figure 4:

Single-cell suspensions were made from B16-OVA tumors of mice treated with PBS or propranolol (Prop). (A) Single-cell suspensions of B16-OVA tumors of PBS- or propranolol-treated mice (CD8+ T cells were pooled from 5 mice/group) were prepared, and tumor infiltrating CD8+ T cells were sorted, lysed, and RNA was extracted for Nanosting nCounter microarray analysis. Graph shows cytokines by log2 (fold-change of PBS/Prop). Blue shows high in PBS group; green shows high in Prop group (B-E) Frequency of (B) IFNγ, (C) TNFα, (D) IFNγ+TNFα+, and (E) IL2 expression in CD8+ T cells from B16-OVA tumors. (F-G) Frequency of (F) IFNγ+TNFα+ and (G) IL2 expression in CD4+ T cells from B16-OVA tumors. n = 5. (B-G) Data are presented as mean±SD; one of two independent experiments; data were analyzed using unpaired Student’s t test, *p<0.05.