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. Author manuscript; available in PMC: 2022 Aug 20.
Published in final edited form as: Free Radic Biol Med. 2021 Jun 3;172:82–89. doi: 10.1016/j.freeradbiomed.2021.05.039

Fig. 4. Gene expression of redox stress response genes to skeletal muscle stimulation.

Fig. 4.

A) Housekeeping gene average Cq change (∆ Cq) in response to skeletal muscle stimulation. B) Housekeeping gene average Cq change (∆ Cq) by group. Based on these results RPL41, RPL27, and RPL711 were selected for housekeeping genes. C) Effects of high intensity (H), low intensity (L) muscle stimulations, and control (C) for target genes HMOX1, NQ01, GCLC, GSR, and NFE2L2 fold change from unstimulated muscle (dotted line). In Nave Controls the mock stimulated muscle (right limb) was compared to the unstimulated control (left limb in all cases). There were no statistically significant increases in gene expression in response to stimulation in either group, although GCLC showed trends for increases regardless of intensity groups. RPL41, RPL27, and RPL711 were used for analysis of target gene fold change. H = High intensity stimulation group, L = Low intensity stimulation group, C = Naïve control group.