Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Experimental Models: Drosophila Strains | ||
| Canton-S | Gero Miesenböck (University of Oxford) | |
| brp MI02987-GFSTF | Bloomington Drosophila Stock Center | RRID:BDSC_59292 |
| R23E10-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_49032 |
| UAS-TrpA1 (II) | Bloomington Drosophila Stock Center | RRID:BDSC_26263 |
| dSyd-1 MI05387-GFSTF | Bloomington Drosophila Stock Center | RRID:BDSC_59414 |
| cacophony sfGFP | Kate O’Connor-Giles (Brown University) | |
| Rim MI03470-GFSTF | Bloomington Drosophila Stock Center | RRID:BDSC_60200 |
| Syt1 MI02197-GFSTF | Bloomington Drosophila Stock Center | RRID:BDSC_59788 |
| Rab3 mCherry | Bloomington Drosophila Stock Center | RRID:BDSC_81509 |
| nSyb GFP | Troy Littleton (MIT) | |
| dlg MI06353-GFSTF | Bloomington Drosophila Stock Center | RRID:BDSC_59417 |
| STaR effector: w−; 20xUAS-RSR.PEST, 79C23S-RSRT-STOP-RSRT-smGFP_V5-2A-LexA/cyo | Orkun Akin (UCLA) | |
| R13F02-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_48571 |
| OK107-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_854 |
| TH-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_8848 |
| R58E02-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_41347 |
| GH146-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_30026 |
| C316-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_30830 |
| MB594B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68255 |
| MB185B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68267 |
| MB371B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68383 |
| MB463B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68370 |
| MB607B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68256 |
| GRASP Effector: w*; P{w[+mC]=lexAop-nSyb-spGFP1-10}2, P{w[+mC]=UAS-CD4-spGFP11}2; MKRS/TM6B | Bloomington Drosophila Stock Center | RRID:BDSC_64315 |
| MB-LexA | Scott Waddell (University of Oxford) | |
| MB543B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68335 |
| R71D08-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_61645 |
| R66C08-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_49412 |
| R25D01-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_49122 |
| R12G04-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_48523 |
| MB434B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68325 |
| MB011B split-Gal4 | HHMI Janelia Research Campus | RRID:BDSC_68294 |
| Antibodies | ||
| Chicken anti-GFP | ThermoFisher | A10262 RRID:AB_2534023 |
| Anti-chicken Alexa488 | ThermoFisher | A11039 RRID:AB_142924 |
| Mouse anti-V5 DyLight550 | BioRad | MCA1360GA RRID:AB_567249 |
| Mouse anti-GFP | Sigma | G6539–100UL RRID:AB_259941 |
| Anti-mouse Alexa488 | ThermoFisher | A11001 RRID:AB_2534069 |
| Software | ||
| Visual Basic sleep analysis macros | Paul Shaw (Washington University in St. Louis) | |
| Prism 8 | Graphpad (www.graphpad.com) | |
| FIJI | FIJI (Imagej.net/FIJI) | |
| Chemicals | ||
| THIP hydrochloride (4,5,6,7-Tetrahydroisoxazolo[5,4-c]pyridin-3-ol hydrochloride) | Sigma-Aldrich | T101-100MG |
| Phosphate buffered saline tablets | Sigma-Aldrich | P4417-100TAB |
| Triton X-100 | Simga-Aldrich | X100-100ML |
| VECTASHIELD Mounting Medium | Vector Laboratories | H-1000 |
| Glyoxal 3% Fixative | Electron Microscopy Sciences | 16525 |
| Paraformaldehyde 16% Solution | Electron Microscopy Sciences | 15710-S |