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. Author manuscript; available in PMC: 2022 Aug 26.
Published in final edited form as: J Biomech. 2021 Jun 17;125:110580. doi: 10.1016/j.jbiomech.2021.110580

Figure 5: Chondrogenic gene expression and Col2a1 immunohistochemistry under free-swelling (FS) or 10% dynamic compression (DC) conditions.

Figure 5:

Samples were analyzed immediately after the final loading cycle in each group for mRNA expression of SOX9 (A), ACAN (B), and COL2A1 (C), calculated as fold change over free-swelling controls, normalized to GAPDH. Data are shown as mean ± s.d. with individual data points. ANOVA with Tukey’s post-hoc tests were used to determine significance (* p < 0.05, ** p < 0.01). Immunohistochemistry for Col2a1 (D) with hematoxylin counterstain (scale bar = 100 μm). N = 4 per group.