| Antibodies |
| Chicken anti-plakoglobin |
Aves Laboratories |
1407 and 1408 |
| Mouse anti-α-tubulin DM1A |
Sigma-Aldrich |
Cat# T6199 RRID:AB_477583 |
| Mouse anti-α-tubulin 12G10 |
Developmental Hybridoma Studies Bank |
RRID: AB_1157911 |
| Rabbit anti-src |
Sigma-Aldrich |
Cat# HPA030875 RRID:AB_2673640 |
| Rabbit anti-fyn |
Sigma-Aldrich |
Cat# HPA023887 RRID:AB_1849226 |
| Mouse anti-yes |
Santa Cruz Biotechnology |
Cat# sc-46674 RRID:AB_627370 |
| Rabbit anti-GAPDH |
Sigma-Aldrich |
Cat# G9545 RRID:AB_796208 |
| Mouse anti-FLAG |
Sigma-Aldrich |
Cat# F7425 RRID: AB_439687 |
| Mouse JL8 anti-GFP |
Takara |
Cat# 632381 RRID:AB_2313808 |
| Mouse P124 anti-desmoglein 1 |
Progen |
Cat# 651111 RRID:AB_1541107 |
| Mouse U100 anti-desmocollin 1a/b |
Progen |
Cat# 65192 RRID:AB_1541099 |
| Mouse anti-vinculin |
Sigma-Aldrich |
Cat# V9131 RRID:AB_477629 |
| Mouse C4 anti-actin |
EMD-Millipore |
Cat# MAB1501 RRID:AB_2223041 |
| Rabbit NW6 and NW161 anti-desmoplakin |
Green Laboratory |
N/A |
| Mouse HECD1 anti-e-cadherin |
M. Takeichi and O. Abe, Riken Center for Developmental Biology |
N/A |
| Rabbit anti-loricrin, rabbit anti-keratin 1, rabbit anti-keratin 5, and rabbit anti-keratin 14 |
J. Segre, NIH |
N/A |
| Rabbit anti-EGF Receptor |
Cell Signaling Technology |
Cat# 4267 RRID:AB_2246311 |
| Rabbit anti-phospho-EGF Receptor Tyr1068 |
Cell Signaling Technology |
Cat# 2234 RRID:AB_331701 |
| Rabbit anti-HER2/ErbB2 |
Cell Signaling Technology |
Cat# 2165 RRID:AB_10692490 |
| Rabbit anti-phospho-HER2/ErbB2 Tyr877 |
Cell Signaling Technology |
Cat# 2241 RRID:AB_2099407 |
| Mouse anti-ZO1 |
BD BioSciences |
Cat# 610967 RRID:AB_398280 |
| Rabbit anti-claudin 1 |
Thermo Fisher Scientific |
Cat# 51-9000 RRID:AB_2533916 |
| Rabbit anti-claudin 4 |
Thermo Fisher Scientific |
Cat# 36-4800 RRID:AB_2533262 |
| Mouse anti-occludin |
Thermo Fisher Scientific |
Cat# 33-1500 RRID:AB_2533101 |
| Rabbit anti-MKL1 |
Sigma-Aldrich |
Cat# HPA030782 RRID:AB_10600930 |
| Secondary goat anti-mouse, -rabbit, and -chicken HRP |
SeraCare Life Sciences |
N/A |
| Secondary goat anti-mouse IgG2a isotype conjugated with Alexa Fluor-488 |
Thermo Fisher Scientific |
Cat# A-21131 RRID:AB_2535771 |
| Secondary goat anti-mouse IgG2a isotype conjugated with Alexa Fluor-647 |
Thermo Fisher Scientific |
Cat# A-21124 RRID:AB_2535766 |
| Secondary goat anti-mouse IgG1 isotype conjugated with Alexa Fluor-488 |
Thermo Fisher Scientific |
Cat# A-21121 RRID:AB_2535764 |
| Secondary goat anti-mouse conjugated with Alexa Fluor-488 |
Thermo Fisher Scientific |
Cat# A-11029 RRID:AB_2534088 |
| Secondary goat anti-mouse conjugated with Alexa Fluor-568 |
Thermo Fisher Scientific |
Cat# A-11004 RRID:AB_2534072 |
| Secondary goat anti-mouse conjugated with Alexa Fluor-647 |
Thermo Fisher Scientific |
Cat# A-32728 RRID:AB_2633277 |
| Secondary goat anti-rabbit conjugated with Alexa Fluor-488 |
Thermo Fisher Scientific |
Cat# A-11008 RRID:AB_143165 |
| Secondary goat anti-rabbit conjugated with Alexa Fluor-568 |
Thermo Fisher Scientific |
Cat# A-11011 RRID:AB_143157 |
| Secondary goat anti-rabbit conjugated with Alexa Fluor-647 |
Thermo Fisher Scientific |
Cat# A-21245 RRID:AB_2535813 |
| Secondary goat anti-chicken conjugated with Alexa Fluor-647 |
Thermo Fisher Scientific |
Cat# A-21449 RRID:AB_2535866 |
| Bacterial and virus strains |
| pLZRS-DPNTP-GFP |
This study |
N/A |
| pLZRS-GFP |
[41] |
N/A |
| pLZRS-Dsg1 |
[41] |
N/A |
| pLZRS-miR Dsg1 |
[41] |
N/A |
| pLZRS–NTshRNA (shCtl) |
[67] |
N/A |
| pLVX myristoylated tdTomato |
Clontech |
N/A |
| Biological samples |
|
|
| Neonatal human epidermal keratinocytes (NHEKs) |
NU SBDRC |
N/A |
| Chemicals, peptides, and recombinant proteins |
| Dispase II |
Roche |
Cat# 04942078001 |
| Goat serum |
Sigma-Aldrich |
Cat# G9023 |
| 4’,6-Diamido-2-Phenylindole (DAPI) |
Sigma-Aldrich |
Cat# D9542 |
| Alexa Fluor-568 Phalloidin |
Thermo Fisher Scientific |
Cat# A12380 |
| Recombinant Human Ephrin-A1 Fc peptide |
R&D Systems |
Cat# 6417-A1-050 |
| Rho Activator I |
Cytoskeleton, Inc |
Cat# CN01 |
| Mubritinib (TAK165) |
Selleck Chemicals |
Cat# S2216 |
| Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
Cat# D8418 |
| PP2 |
Sigma-Aldrich |
Cat# P0042 |
| CCG-1423 |
Sigma-Aldrich |
Cat# 555558 |
| Ingenio Electroporation Solution |
Mirus Bio |
Cat# MIR 50117 |
| Polybrene |
Sigma-Aldrich |
Cat# H9268 |
| Albumin from bovine serum (BSA) |
Sigma-Aldrich |
Cat# A7906 |
| 16% Formaldehyde (w/v), Methanol-free |
Thermo Fisher Scientific |
Cat# PI28908 |
| Urea |
Thermo Fisher Scientific |
Cat# BP169-212 |
| Sodium Dodecyl Sulfate (SDS) |
Thermo Fisher Scientific |
Cat# BP166-500 |
| Tris Hydrochloride |
Thermo Fisher Scientific |
Cat# BP153-500 |
| β-Mercaptoethanol |
Sigma-Aldrich |
Cat# M6250 |
| Acrylamide/Bis-acrylamide, 30% solution |
Sigma-Aldrich |
Cat# A3574 |
| Xylene |
Thermo Fisher Scientific |
Cat# X3P |
| Citric acid |
Sigma-Aldrich |
Cat# C0759 |
| Glycine |
Sigma-Aldrich |
Cat# G8790 |
| Methanol |
Sigma-Aldrich |
Cat# 179337 |
| Glycerol |
Thermo Fisher Scientific |
Cat# BP229 |
| Ethyl Alcohol, 200 proof |
Thermo Fisher Scientific |
Cat# A4094 |
| Triton X-100 |
Sigma-Aldrich |
Cat# X100 |
| Tween20 |
Sigma-Aldrich |
Cat# P1379 |
| ProLong Gold antifade reagent |
Thermo Fisher Scientific |
Cat# P36930
|
| CELLstart CTS Substrate |
Thermo Fisher Scientific |
Cat# A10142-01 |
| Experimental models: Cell lines |
| Phoenix cells |
G. Nolan, Stanford University |
N/A |
| Medium 154CF, Kit (M154) With gentamicin/amphotericin B solution and CaCl2 |
Thermo Fisher Scientific |
Cat# M154CF500 |
| Human Keratinocyte Growth Supplement (HKGS) Kit |
Thermo Fisher Scientific |
Cat# S001K |
| PBS |
Thermo Fisher Scientific |
Cat# 21040CV |
| Opti-MEM™ I Reduced Serum Medium |
Thermo Fisher Scientific |
Cat# 11058021 |
| PCT Epidermal Keratinocyte Medium |
Zenbio |
Cat# CnT-07 |
| CnT-Prime 3D Barrier Media for 3D Epidermal Models |
Zenbio |
Cat# CnT-PR-3D |
| Oligonucleotides |
| siGENOME Non-Targeting siRNA Control Pool #2 |
Dharmacon |
Cat# D-001206-14 |
| siGENOME SMARTpool DSP siRNA |
Dharmacon |
Cat# D-019800-17 |
| stealth DSP siRNA oligonucleotide 5’-CAGGGCUCUGUCUUCUGCCUCUGAA-3’ |
Thermo Fisher Scientific |
N/A |
| Software and algorithms |
| ZEN 2.3 software |
Carl Zeiss |
RRID:SCR_013672 |
| NIS-Elements |
Nikon |
RRID:SCR_014329 |
| ImageJ |
NIH |
RRID:SCR_003070 |
| Prism |
GraphPad |
RRID:SCR_002798 |
| Microsoft Excel |
Microsoft |
RRID:SCR_016137 |
| Photoshop |
Adobe |
RRID:SCR_014199 |
| Illustrator |
Adobe |
RRID:SCR_010279 |
| Other |
| 24 mm Transwell 0.4 μm pore inserts |
Costar |
Cat# 3450 |
| Nucleofector 2b Device |
Lonza |
Cat# AAB-1001 |
| Amicon® Ultra-15 Centrifugal Filter Unit |
Sigma-Aldrich |
Cat# UFC9030 |
| EVOM epithelial volt/ohm meter |
World Precision Instruments |
N/A |
