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. 2021 Aug 10;12:4814. doi: 10.1038/s41467-021-25079-4

Fig. 3. Glutamine is sufficient to sustain ATP levels, but not to activate mTORC1.

Fig. 3

A ATP/ADP ratio of amino acid-starved U2OS cells incubated in the presence or absence of LQ with or without BPTES or DON during 72 h. B, C GLS or GDH expressions were knocked down using small interfering RNA (siRNA) in U2OS cells for 48 h. Cells were then treated with LQ for 72 h and the ATP/ADP ratio was measured. Scramble non-targeting siRNA was used as a control. Immunoblots of GLS or GDH levels are presented as a control of the knockdown. D ATP/ADP ratio of amino acid-starved U2OS cells treated with LQ or DMKG for 72 h. E, F Immunoblot of amino acid-starved U2OS cells treated with or without LQ in combination with BPTES (E) or DON (F) for 72 h. Activity markers of AMPK and mTORC1 were analysed. Graphs show mean values ± SEM (n = 3 biologically independent experiments). *p < 0.05 (ANOVA analysis followed by a post hoc Bonferroni test). Source data are provided as a Source Data file.