Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Purified anti-E. coli RNA Polymerase α Antibody | Biolegend | Cat #663104 |
| Rabbit polyclonal anti-CtrA | Lab collection | Cat #: N/A |
| Rabbit polyclonal anti-GcrA | Lab collection | Cat #: N/A |
| Rabbit polyclonal anti-SciP | Lab collection | Cat #: N/A |
| Bacterial strains | ||
| Caulobacter crescentus synchronizable wild-type CB15N (NA1000) | Lab collection | ML76 |
| CckA-eGFP:gent | (Chen et al., 2011) | ML1681 |
| DivL-eGFP:gent | (Chen et al., 2011) | ML1756 |
| PlacI-lacI (hfaA locus); Plac-dnaA (dnaA locus) | (Badrinarayanan et al., 2015) | ML2000 |
| MipZ-CFP:gent | (Badrinarayanan et al., 2015) | ML2395 |
| MipZ-CFP:kan | (Badrinarayanan et al., 2015) | ML2397 |
| popZ::pBGent-PopZ-eYFP | (Ebersbach et al., 2008) | ML1900 |
| Pxyl-gcrA-3xflag | (Haakonsen et al., 2015) | ML2297 |
| van::Pvan-1Xflag-parB | (Tran et al., 2018) | ML3243 |
| van::Pvan-1Xflag-parB(G101S) | (Tran et al., 2018) | ML3244 |
| BTH101 E. coli | Euromedex | ML1726 |
| See Table S1 for a complete list of the bacterial strains constructed for this study | See Table S1 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Blue loading Buffer pack | NEB | Cat #: B7703S |
| Carbenicillin | Goldbio | Cat #: C-103-100 |
| Chloramphenicol | Sigma | Cat #: 60378 |
| dNTP solution mix | NEB | Cat #: N0447L |
| DEPC-Treated Water | Thermo Fisher | Cat #: AM9906 |
| DpnI | NEB | Cat #: R0176L |
| Ethidium Bromide | Sigma | Cat #: E1510 |
| 16% Paraformaldehyde Aqueous Solution | Fisher Scientific | Cat #: 50-980-487 |
| Gentamycin | Sigma | Cat #: G3632 |
| Gibson 2X Master Mix | NEB | Cat #: E2611L |
| GlycoBlue | Ambion | Cat #: AM9516 |
| Kanamycin | Sigma | Cat #: K1377 |
| Kapa HiFi HotStart DNA Polymerase | Kapa Biosystems | Cat #: KK2502 |
| Kapa 2X qPCR Master Mix | Kapa Biosystems | Cat #: KK4609 |
| NdeI | NEB | Cat #: R0111S |
| NEBuilder® HiFi DNA Assembly Master Mix | NEB | E2621L |
| NheI-HF | NEB | Cat #: R3131M |
| Oxytetracycline | Sigma | Cat #: T3383 |
| Percoll | GE Healthcare | Cat #: 17-0891-01 |
| Random Primers | Invitrogen | Cat #: 48190-011 |
| RNase A | QIAGEN | Cat #: 19101 |
| RNase H | NEB | Cat #: M0297L |
| Superase-In | ThermoFisher | Cat #: AM2696 |
| Superscript III | ThermoFisher | Cat #: 18080093 |
| SYTOX Green Nucleic Acid Stain | Invitrogen | Cat #: S7020 |
| T4 DNA Ligase | NEB | Cat #: M0202L |
| Trizol | ThermoFisher | Cat #: 15596026 |
| Texas Red™-X, Succinimidyl Ester, single isomer | ThermoFisher | Cat #: T20175 |
| Turbo DNase | Ambion | Cat #: AM2238 |
| Critical Commercial Assays | ||
| Direct-zol RNA MiniPrep | Zymo Research | Cat #: R2050 |
| ZR Plasmid Miniprep-Classic | Zymo Research | Cat #: D4015 |
| DNA Clean & Concentrator-25 (Capped) | Zymo Research | Cat #: D4034 |
| Zymoclean Gel DNA Recovery Kit (capped) | Zymo Research | Cat #: D4008 |
| QIAGEN Gel Extraction Kit | QIAGEN | Cat #: 28706 |
| QIAGEN PCR Purification Kit | QIAGEN | Cat #: 28106 |
| QIAGEN Plasmid Mini Kit | QIAGEN | Cat #: 27106 |
| SuperSignal West Femto Maximum Sensitivity Substrate | ThermoFisher | Cat #: 34095 |
| Oligonucleotides | ||
| For primer table, see Table S2 | Integrated DNA Technologies (IDT) | https://sg.idtdna.com/ |
| Recombinant DNA | ||
| pJS14 PxylX::ctrA | (Domian et al., 1997) | ML1500 |
| PxylX::ctrAΔ3Ω | (Domian et al., 1997) | ML46 |
| pBVMCS-4-PA5295 | (Duerig et al., 2009) | ML2681 |
| pQF | (Kaczmarczyk et al., 2013) | ML3414 |
| See Table S3 for a complete list of the plasmids constructed for this study | N/A | N/A |
| Software and Algorithms | ||
| Fiji/ImageJ | (Schindelin et al., 2012) | https://imagej.net/Welcome |
| FlowJo (X 10.0.7r2) | FlowJo LLC, 2013-2017 | https://www.flowjo.com/ |
| Metamorph | Molecular Devices, 2017 | https://www.moleculardevices.com/systems/metamorph-research-imaging/metamorph-microscopy-automation-and-image-analysis-software |
| MicrobeJ | (Ducret et al., 2016) | http://www.microbej.com/ |
| Prism 9.0.1 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Others | ||
| 35 mm Glass bottom dish | Cellvis | Cat #: D35-20-0-N |