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. 2021 Aug 10;6:44. doi: 10.1038/s41536-021-00154-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative immunofluorescence staining of NRCMs exposed to 6 h hypoxia and cocultured with different patches was observed with α-actinin antibody (green), DAPI (blue), and TUNEL (red). Scale bars represented 50 μm. b Statistical analysis of TUNEL-positive cell counts per 100 cells. n = 5/group. c Representative flow cytometric Annexin V FITC-A vs Propidium Iodide-A quad plots of NRCMs after 6 h hypoxia treatment. d The percentage of Annexin V−/PI− cell were summarized. n = 3/group. e Representative wound healing assay image of cardiac fibroblasts cocultured with different patches after 24 h. f Statistical analysis of healed wound area difference between two groups. n = 5/group. Data were represented as the mean ± SEM. Two group comparisons were analyzed by the unpaired Student’s t-test; **P < 0.01, ****P < 0.0001.