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. 2021 Aug 10;6:44. doi: 10.1038/s41536-021-00154-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — H&E stained section (a), Masson’s trichrome staining (b) and Sirius Red staining (c) of mice hearts at low magnification after heart was obtained 28 days after MI. Scale bars represented 500 μm. d Brain natriuretic peptide (BNP) immunohistochemical staining of hearts from PLLA, intramyocardial injection (IM) and PLLA-HA/ACE2 treatment group 28 days after MI. Scale bars represented 40 μm. Morphometric parameters including the percentage of infarct size percentage of total LV area (e) and the percentage of infarcted thickness of LV anterior wall (f) were measured from the Sirius Red stained slides via ImageJ software. n = 5/group. g The integrated optical density (IOD)/area ratios of BNP were quantified from the immunohistochemical stain by Image-Pro Plus software. n = 5/group. Data were represented as the mean ± SEM and was analyzed for statistical significance using one-way ANOVA followed by Tukey’s multiple comparison test; NS no significant difference, *P < 0.05, ****P < 0.0001.