Table 3.

Number of directly inoculated or contact exposed Pekin ducks or guinea fowl shedding virus detected by real-time RT-PCR, percent of birds infected within dose groups for each isolate, mean bird infectious dose (calculated from the inoculated birds), and seroconversion.

			Inoculated birds					Contact exposed birds

Species	Isolate	Dose^a	Virus shed^b			% infected^d		Virus shed^b			% infected
			OP	CL	Serology^c	% infected^d	BID^e	OP	CL	Serology	% infected
Pekin duck	Ck/NY/042097/18	6	2/5	0/5	2/5	40	> 6.0	0/3	0/3	0/3	0
		4	2/5	1/5	0/5	40		0/3	0/3	0/3	0
		2	0/5	0/5	0/5	0		0/3	0/3	0/3	0
	Ck/NY/2019	6	4/5	4/5	5/5	100	3.3	3/3	1/3	3/3	100
		4	2/5	3/5	4/5	80		0/3	3/3	3/3	100
		2	0/5	0/5	0/5	0		0/3	0/3	0/3	0
Guinea Fowl	Ck/NY/042097/18	6	13/13	12/13	12/13	100	4.3	3/3	3/3	3/3	100
		4	2/5	0/5	1/5	40		0/3	0/3	0/3	0
		2	1/5	0/5	1/5	20		0/3	0/3	0/3	0
	Ck/NY/2019	6	13/13	11/13	13/13	100	2.8	3/3	2/3	3/3	100
		4	5/5	4/5	5/5	100		3/3	3/3	3/3	100
		2	0/5	0/5	1/5	20		0/3	0/3	0/3	0

^a.

Log10 50% egg infectious dose per bird.

^b.

Number of birds shedding virus / total number of birds

^c.

Number of birds positive for antibody / total number of birds

^d.

Birds were considered infected if viral RNA was detected in oropharyngeal (OP) and cloacal (CL) swabs at any time point and/or the chickens was positive for antibodies by hemagglutination inhibition assay at 14 days post inoculation.

^e.

Log10 50% bird infectious dose (BID) per mL.