Table 4.

Nonsynonymous amino acid substitutions found in the Ck/NY/19 isolate when compared with other challenge viruses from this study. Notable substitutions (K350R, A85T, I432V) and the deletion in the NA stalk deletion region are bolded.

Protein	Position	Ck/NY/2019	Other challenge viruses	Reference(s)
PB2	214	R	K
PB1	350	K	R	(Suzuki et al., 2009)
PA	85	A	T	(Kajihara et al., 2013)
	311	I	M
	432	I	V	(Bogs et al., 2010)
	504	V	I
	626	K	R
PA-X	85	A	T
	215	L	P
HA	109	N	S
	126	L	F
	226	V	E
	228	V	A
	322	T	S
	334	A	V
	406	I	V
	429	I	L
NP	357	K	Q
NA	63–82 (Stalk region)	Deleted	TEIVYLNNTTIEREICPKAV	(Banks et al., 2001; Hossain et al., 2008; Munier et al., 2010; Sorrell et al., 2010)
M1	107	V	I
M2	27	V	I
NS1	75	K	E
NEP	111	G	S