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. 2021 Aug 10;12:4813. doi: 10.1038/s41467-021-25102-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a RNA-seq was performed on sorted CD11c⁺ and CD11c⁻ PB/PCs (CD138⁺TACI⁺) from aged (24+wk) female DKO (F) mice. Volcano plot showing differentially expressed genes (p < 0.01 after Benhamini-Hochberg false discovery rate (FDR) was used to correct for multiple comparisons) between CD11c⁺ and CD11c⁻ PB/PCs. Blue dots indicate ABC target genes upregulated in CD11c⁺ PB/PCs. b Plot showing the enrichment of the ABC geneset from DKO mice in CD11c⁺ PB/PCs. c Representative histograms and quantifications of Fcrl5, Cxcr3, and Tbet in CD11c⁺ PB/PCs (CD19^mid/loCD138⁺; red) and CD11c⁻ PB/PCs (black) from aged (24+wk) DKO(F) mice. CD19⁺CD11c⁺CD11b⁺ (ABCs) (green) and CD19⁺GL7⁻Fas⁻ cells (gray) are shown as controls. Data show mean ± SEM; n = 6 for Tbet, n = 8 for CXCR3, n = 9 for FcRL5 over 3–5 independent experiments; p-value by paired two-tailed t-tests. d Plots showing the enrichment of Blimp1-induced⁷⁴ and IRF4-induced target genes in CD11c⁻ PB/PCs by GSEA. e Representative histograms and quantifications of B220, MHC-II, and Ki67 expression in the indicated populations from aged (24+wk) DKO(F) mice. Data show mean ± SEM; n = 4 for Ki67, n = 5 for B220, n = 7 for MHC-II over 2–3 independent experiments; p-value by paired two-tailed t-tests. f Representative RT-qPCR showing Ciita expression in CD11c⁺ or CD11c⁻ PB/PCs sorted from aged (24+wk) DKO(F) mice as in Fig. 6a. Data representative of 3 independent experiments and show mean ± SD; n = 3; p-value by unpaired two-tailed t-test. g Plot showing the top pathways enriched in CD11c⁺ PB/PCs as compared to CD11c⁻ PB/PCs by GSEA. Dotted line indicates significance threshold at FDR q < 0.25. h, i Heatmap showing the expression of migratory (h) and inflammatory target genes (i) that are differentially expressed (p < 0.01) between CD11c⁺ and CD11c⁻ PB/PCs in DKO(F) mice from Fig. 6a. j Representative RT-qPCR showing Il1b expression in CD11c⁺ or CD11c⁻ PB/PCs sorted from aged (24+wk) DKO(F) mice as in Fig. 6a. Data representative of 3 independent experiments and show mean ± SD; n = 3; p-value by unpaired two-tailed t-test.