Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 10;12:4813. doi: 10.1038/s41467-021-25102-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a–c Quantifications showing the numbers of CD11c⁺Tbet⁺ ABCs (a), CD11c⁺CD11b⁺ ABCs (b), and total GL7⁺Fas⁺ B cells (c) from the spleens of aged (24+wk) female C57BL/6 (WT), female DKO(F), female CD23-Irf8.DKO(F), female CD23-Irf5^f/+.DKO(F), and female CD23-Irf5^f/f.DKO(F) mice. Data show mean ± SEM; n = 4 for WT, n = 5 for DKO(F), CD23-Irf5^f/+.DKO(F), and CD23-Irf5.DKO(F), n = 7 for CD23-Irf8.DKO(F), n = 12 for DKO(F) over 6–7 independent experiments; p-value by 1-way ANOVA followed by Tukey’s test for multiple comparisons. d Representative immunofluorescence images of B220 (blue), GL7 (pink), and CD3 (green) on the indicated mice. Data representative of 4 mice per genotype. Bars show 50 μm. e, f Quantifications showing the numbers of total PB/PCs (e; B220^mid/loCD138⁺) and CD11c⁺ PB/PCs (f) from the indicated aged (24+wk) mice. Data show mean ± SEM; n = 5/6 for WT, n = 5/13 for DKO(F), n = 7 for CD23-Irf8.DKO(F), n = 5 for CD23-Irf5^f/+ (e); n = 3/5 for WT, n = 5/12 for DKO(F), n = 7 for CD23-Irf8.DKO(F), n = 5 for CD23-Irf5^f/+ (f) over 6–7 independent experiments; p-value by 1-way ANOVA followed by Tukey’s test for multiple comparisons. g ELISA data showing anti-dsDNA IgG2c in the serum from the indicated aged (24+wk) mice. Data show mean ± SEM; n = 4/8 for WT, n = 4/14 for DKO(F), n = 5 CD23-Irf8.DKO(F), n = 7 for CD23-Irf5^+/-.DKO(F), n = 6 for CD23-Irf5^f/f.DKO(F); p-value by 1-way ANOVA followed by Tukey’s test for multiple comparisons. h–k CD23⁺ B cells from the indicated young (8–12wk) mice were stimulated for 2–3d with αIgM, αCD40, and IL-21. h Quantification of CD11c⁺Tbet⁺ B cells after 3d culture. Data show mean ± SEM; n = 7 for WT, DKO(F), and CD23-Irf8.DKO(F), n = 3 for CD23-Irf5.DKO(F); p-value by 1-way ANOVA followed by Tukey’s test for multiple comparisons. i Quantifications showing the expression of CD11c and Tbet in the indicated strains after 3d culture as in Fig. 4h. Data show mean ± SEM; n = 6 for WT and DKO(F), n = 5 for CD23-Irf8.DKO(F), n = 4 for CD23-Irf5.DKO(F); p-value by 1-way ANOVA followed by Tukey’s test for multiple comparisons. j ELISA data showing CXCL10 production in the supernatants after 3d culture as in Fig. 4h. Data representative of 3 independent experiments and show mean ± SD. k Representative ChIP-qPCR of IRF8 and IRF5 binding at the Cxcl cluster in the indicated young (8–12wk) mice. Data representative of 3 independent experiments and show mean ± SD.