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. 2021 Aug 10;11:16194. doi: 10.1038/s41598-021-95435-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zonal distance distribution of RNA-seq derived cluster and single hairpin units. Bars show the distribution of RNA-seq derived hairpin units with respect to distance from pos^st for the cluster (a) and single (b) units as per in zone location. The different window sizes (represented by different colors) show the different distances from pos^st and their corresponding percentages on the y-axis. The total IRs from which percentage has been calculated for each of the genomes A–M on the x-axis are given on top of each bar for Zone 2 and at bottom of each bar for Zone 1. The x-axis labels show the 13 analyzed genomes, as indicated in Table 1. The total number of hairpins contributing to each bar is shown on the top/bottom of each bar. There are 111 transcription units where the hairpin is located > 75 bases from the pos^st; these are not shown in the plot. (c) For shortest distance calculation, if hairpin (hp) is in Zone 1 (between IR start and pos^st), then n is calculated as the distance between the last hairpin in cluster unit (last blue line in hp1 cluster) to pos^st. So d1 in the image is ‘n’ for zone 1 hairpins. If hairpin in Zone 2 (between pos^st and pos^stp), then n is calculated as the distance between pos^st and the first hairpin of cluster unit (first blue line in hp2 cluster). So d2 in the image is ‘n’ for Zone 2 hairpins. (d) The cluster hairpin hp3 lies in Zone 1 and Zone 2. In such a case, we assign n = 0.