Table 1.

Organism list used in our study.

	Organism name	Bacterial phyla	GC%	NCBI ID	RNA-seq ID
A	Staphylococcus aureus Newman	Firmicutes	32.9	NC 009641	ERR1337989-91
B	Clostridium phytofermentans		35.4	NC 010001	ERR1709598
C	Myobacterium gilvum	Actinobacteria	67.9	NC 009338	SRR748310
D	Bifidobacterium pseudocatenulatum		56.4	AP012330	ERR2789029-30
E	Treponema denticola	Spirochaetes	37.9	NC 002967	ERR2177101-02
F	Leptospira interrogans		34.9	LT962963	SRR3222245-46, 49
G	Fusobacterium nucleatum	Fusobacteria	27.2	NC 003454	ERR2177097
H	Nostoc punctiforme	Cyanobacteria	41.4	NC 010628	SRR5197990
I	Prochloroccus. marinus		50.7	NC 005071	ERR2137696-98
J	Nodularia spumigena		41.3	CP020114	SRR5801900-01
K	Pseudomonas aeruginosa	Proteobacteria	66.6	NC 002516	SRR4237919-20
L	Syntrophus aciditrophicus		51.5	NC 007759	SRR3944268
M	Klebsiella pneumoniae		57.45	CP011985	SRR3018810,12

List of phyla and organisms used for validation of our method.