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. Author manuscript; available in PMC: 2022 Aug 10.
Published in final edited form as: Curr Biol. 2021 Jun 4;31(15):3207–3220.e4. doi: 10.1016/j.cub.2021.05.010

Figure 2. Actin binding and microtubule interacting domains of Shot are essential for oocyte growth.

Figure 2.

(A) Diagrams of the full-length Shot and truncated mutants. (B) Summary of Orb and phalloidin staining phenotypes in control (mat αtub-Gal4[V37]/+), shotRod-RNAi (mat αtub-Gal4[V37]/UASp-shotRod-RNAi), and shotRod-RNAi +shotARod-GFP (UASt-shot.L(A)ΔRod-GFP/+; mat αtub-Gal4[V37]/UASp-shotRod-RNAi). (C) A schematic illustration of knockdown of wild-type Shot by shot-RNAi in shot truncated mutant heterozygous background. KD, knockdown. (D) Summary of Orb and phalloidin staining in listed phenotypes. Unlike one copy of shotWT, one copy of shotΔABD or shotΔEGC is unable to drive normal oocyte growth.