REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Orb monoclonal (mouse)	Developmental Studies Hybridoma Bank	orb 6H4
Anti-Shot monoclonal (mouse)	Developmental Studies Hybridoma Bank	mAbRod1
FITC-conjugated anti-β tubulin monoclonal (mouse)	ProteinTech	Cat#: CL488–66240
FITC-conjugated anti-mouse IgG antibody (Goat)	Jackson ImmunoResearch Laboratories, Inc	Cat# 115–095–062
TRITC-conjugated anti-mouse IgG antibody (Goat)	Jackson ImmunoResearch Laboratories, Inc	Cat# 115–025–003
Chemicals, peptides, and recombinant proteins
Rhodamine Phalloidin	Thermo Fisher Scientific	Cat # R415
DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride)	Thermo Fisher Scientific	Cat# D1306
16% Paraformaldehyde aqueous methanol-free solution, EM Grade	Thermo Fisher Scientific (Manufacturer: Electron Microscopy Sciences)	Cat# 50–980–488
Halocarbon oil 700	Sigma-Aldrich	Cat# H8898–50ML
Triton X-100	Fisher Scientific	Cat# BP151–500
BSA Bovine Serum Albumin (BSA)	DOT Scientific	Cat# DSA30075–100
Normal Goat Serum	SouthernBiotech	Cat# 0060–01
Nutri-Fly® Bloomington Formulation	Genesee	Cat# 66–121
Experimental models: organisms/strains
Drosophila melanogaster: hs-FLP	Bloomington stock center	RRID:BDSC_1929
Drosophila melanogaster: FRTG13	Bloomington stock center	RRID:BDSC_1956
Drosophila melanogaster: FRTG13 shot [3]	Bloomington stock center	RRID:BDSC_5141
Drosophila melanogaster: FRTG13 ubi-GFP.nls	Bloomington stock center	RRID:BDSC_5826
Drosophila melanogaster: mat αtub-Gal4 [V37]	Bloomington stock center	RRID:BDSC_7063
Drosophila melanogaster: UAS-shotRod-RNAi (TRiP.GL01286)	Bloomington stock center	RRID:BDSC_41858
Drosophila melanogaster: UASt-shot.L(A)Δrod-GFP	Bloomington stock center	RRID:BDSC_29040
Drosophila melanogaster: shot ΔABD	Bloomington stock center	RRID:BDSC_10522
Drosophila melanogaster: UASp-LifeAct-TagRFP	Bloomington stock center	RRID:BDSC_58713; RRID:BDSC_58714
Drosophila melanogaster: UASp-RFP-Golgi	Bloomington stock center	RRID:BDSC_30908
Drosophila melanogaster: UASp-F-Tractin-tdTomato	Bloomington stock center	RRID:BDSC_58989
Drosophila melanogaster: UAS-Dhc64C-RNAi (TRiP.GL00543)	Bloomington stock center	RRID:BDSC_36583
Drosophila melanogaster: shot ΔEGC	Gift from Dr. Ferenc Jankovics	[20]
Drosophila melanogaster: ubi-GFP-Pav	Gift from Dr. David Glover	[36]
Drosophila melanogaster: nos-Gal4-VP16	Gift from Dr. Edwin Ferguson lab	[70,71]
Drosophila melanogaster: mat αtub-RFP-Staufen	Gift from Dr. Daniel St Johnson	[45]
Drosophila melanogaster: UASp-GFP-LD	Gift from Dr. Michael Welte	[46]
Drosophila melanogaster: UASp-GFP-Patronin	Gift from Dr. Uri Abdu	[31, 51, 69]
Drosophila melanogaster: UASp-EB1-GFP	Gift from Dr. Antoine Guichet	[52]
Drosophila melanogaster: ubi-EB1-GFP	Gift from Dr. Derek Applewhite	[53, 72]
Drosophila melanogaster: UASp-Staufen-SunTag	Generated in our lab	[69]
UAS-shotABD-RNAi	This paper	N/A
UAS-shot EGC -RNAi	This paper	N/A
UASp-Mtio-MoxMaple3	This paper	N/A
Recombinant DNA
Plasmid: pWalium22-shotABD-RNAi	This paper	N/A
Plasmid: pWalium22-shotEGC-RNAi	This paper	N/A
Plasmid: pUASp-UASp-Mito-MoxMaple3	This paper	N/A
Software and algorithms
NIS-Elements	Nikon Instruments Inc.	https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research
FIJI	open source[80]	https://imagej.net/Fiji