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. 2021 Aug 10;11:16193. doi: 10.1038/s41598-021-95607-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sensitivity and specificity of improved RT-LAMP assay. (a) Determination of 50% endpoints for O117_Q and O117_N preparations of RT-LAMP assay. Full length transcripts were serially diluted in buffer AVE to achieve the total RNA input/reaction indicated on the y axis. Each dot represents one experimental replicate. Dashed lines indicate 50% endpoint as calculated by the Reed-Muench method. Kits were dried except where indicated for RNA Control 2. Full length transcript indicated on figure. Dried reaction mix containing (b) O117_N and (c) O117_Q were tested against non-SARS human-infective coronaviruses. A and B represent two technical replicates.