Table 2.
Comparison of EDE complement factor concentrations between groups.
| Complement factors | ANCOVA | Effect Sizes | ROC | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pathway | Molecules | Functions | R2 adjusted | Prob > F | AICc | BIC | Group p-value | Beta | Cohen's d (95% CI) | FDR: p-Value Adj | AUC (95% CI range) |
| C1q | Antigen or Ab complex binding | 0.60 | < 0.0001 | 40 | 43 | 0.002 | 0.59 | 1.18 (0.43–1.91) | 0.004 | 0.94 (0.84–1.00) | |
| C4b | Pathogen/cell binding for opsonization | 0.03 | 0.20 | 58 | 60 | 0.60 | 0.12 | 0.22 (− 0.73 to 1.16) | 0.7 | 0.64 (0.41–0.87) | |
| C3b | Pathogen/cell binding for opsonization; amplification via alternative pathway; C5 binding in prep for cleavage | 0.67 | < 0.0001 | 40 | 42 | < 0.0001 | 0.72 | 1.60 (0.78–2.40) | 0.0003 | 0.99 (0.97–1.00) | |
| C5b-9 | Membrane attack complex | 0.68 | < 0.0001 | 25 | 28 | 0.0002 | 0.67 | 1.09 (0.49–1.67) | 0.0008 | 0.98 (0.92–1.00) | |
| Bb | Component of C3bBb “classical” convertase | 0.71 | < 0.0001 | 49 | 52 | < 0.0001 | 0.80 | 2.31 (1.25–3.33) | 4.0E−05 | 0.99 (0.96–1.00) | |
| Factor B | Bb precursor; Activating Enzyme | 0.74 | < 0.0001 | 28 | 31 | < 0.0001 | 1.04 | 2.14 (1.32–2.93) | 5.0E−07 | 0.94 (0.82–1.00) | |
| Factor D | B activating/cleaving enzyme | 0.02 | 0.30 | 34 | 37 | 0.10 | 0.40 | 0.53 (− 0.11 to 1.15) | 0.2 | 0.74 (0.53–0.94) | |
| CD59 | Prevents formation of MAC | 0.67 | < 0.0001 | 57 | 60 | < 0.0001 | − 0.92 | 2.93 (1.65–4.17) | 2E−05 | 0.99 (0.97–1.00) | |
| Factor I | Protease cleaves C3b | 0.19 | 0.03 | 49 | 52 | 0.02 | − 0.63 | 1.15 ( 0.27–2.01) | 0.02 | 0.74 (0.54–0.94) | |
| CR1 | Binds Bb & displaces C3b | 0.002 | 0.40 | 40 | 43 | 0.60 | − 0.13 | 0.22 (− 0.46 to 0.90) | 0.6 | 0.50 (0.28–0.73) | |
| DAF | Displaces Bb from C3b | 0.03 | 0.30 | 14 | 17 | 0.10 | − 0.42 | 0.36 (− 0.05 to 0.78) | 0.1 | 0.64 (0.42–0.87) | |
| CD46 | Factor I co-factor | 0.09 | 1.0 | 40 | 43 | 0.90 | − 0.03 | 0.03 (− 0.64 to 0.70) | 0.9 | 0.53 (0.30–0.76) | |
| MBL | Mannose or bacteria binding | 0.02 | 0.80 | 61 | 64 | 0.50 | − 0.18 | 0.44 (− 0.59 to 1.47) | 0.5 | 0.59 (0.34–0.86) | |
This table includes a list of complement factors within pathways and respective functions, and results from statistical models: ANCOVA adjusted for age; Effect sizes (Cohen’s d) based on standard least square models; AUC based from ROC analyses.
Statistically significant values are highlighted in bold. For ANCOVA, only statistically significant results are provided. P values are rounded to one non-zero digit.
R2 adjusted for age; CI confidence interval.