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. 2021 Aug 10;12:4821. doi: 10.1038/s41467-021-25101-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Summary of the HTLV-1/ATL panel. b Bar graph shows target gene coverage per 100 million reads in different sequencing platforms. c Venn diagrams depict overlap between detected SNVs by WGS, WES, and panel-based Target-seq in MT-2 (left) and TL-Om1 (right). d, e Bar graphs show proportion of recalled SNVs (d) and precision rates (e) by panel-based deep sequencing from serial diluted samples. SNVs detected by WGS were used as reference. f Scatter plots show observed VAF (x-axis) and expected VAF (y-axis) of detected SNVs in MT-2 (upper) and TL-Om1 (lower). The expected values were calculated from WGS and WES dataset diluted to 100% (black), 50% (dark gray), 20% (gray), 10% (blue), 5% (orange), and 1% (green). Correlation coefficients (R) are provided in the graphs.