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. 2021 Aug 10;12:4821. doi: 10.1038/s41467-021-25101-9

Fig. 2. Performance evaluation of HTLV-1/ATL panel for provirus sequencing and clonotyping.

Fig. 2

a Bar graph shows provirus coverage per 100 million reads in different sequencing platforms. N/A not applicable. b IGV images depict sequence reads mapped to whole HTLV-1 provirus by panel-based deep sequencing and WGS in MT-2 cells. Internal region with low coverage means defective region. c Venn diagrams depict overlap between detected provirus SNVs from WGS and panel-based deep sequencing in MT-2 (upper) and TL-Om1 (lower). d A schematic view illustrates clonality assessment by soft-clipped host–virus junction reads. e IGV images depict VIS reads called as a dominant clone (chr1:121,509,424) in TL-Om1 by WGS, WES, and panel-based Target-seq (100, 50, and 10% dilution). f Venn diagrams depict overlap between detected unique VISs from WGS, WES, and panel-based deep sequencing. g Scatter plots show observed clonality (x-axis) and expected clonality (y-axis). The expected values were calculated from Target-seq (100%) VIS support tags diluted to 100% (black), 50% (dark gray), 20% (gray), 10% (blue), 5% (orange), and 1% (green). Correlation coefficients (R) are provided in the graphs. h Stacked graph shows comparison of clone sizes calculated by VIS support tags in diluted samples and WGS.