Fig. 3. Nek1 deficiency disrupts proteome homeostasis in the CNS.

a, b Cerebral cortical sections from mice (3 weeks old) with indicated genotypes were stained with Thioflavin S for protein aggregates and quantified (b). Mean ± SD. n = 4 sections. One-way ANOVA with Dunnett’s test. Bar = 50 µm. c, d Cerebral cortical sections of mice (3 weeks old) with indicated genotypes were immunostained with anti-α-synuclein(red) and anti-NeuN(green); and the fluorescent intensity of α-synuclein in each NeuN positive cells was quantified (d). Bar = 50 µm. n = 3 sections per group. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s test. e Total cortical lysates from Nek1^+/+ mice, Nek1^Kat2J/Kat2J mice, Nek1^+/+; Ripk1^D138N/D138N mice and Nek1^Kat2J/Kat2J;Ripk1^D138N/D138N mice were analyzed by western blotting with indicated antibodies. Uncropped blots in the Source Data file. f, g The mRNAs from spinal cords (f) or brains (g) mice with indicated genotypes (n = 3 per group, 3 weeks old) were extracted and quantified for the levels of indicated cytokines by qPCR. Data are presented as mean ± SD. One-way ANOVA with Dunnett’s test.