Fig. 4.

Initial screening of the post-thawing viability, using the MTS assay, after cryopreservation with 10% (v/v) Me₂SO or 10% (w/v) NADES. A. in L929 cells. Data represent means ± SD (n = 2). Statistically significant differences were determined by Dunnett's multiple comparisons test and are represented in asterisks: *p < 0.05; ***p < 0.005 and ****p = 0.0001, two-way ANOVA. The absence of asterisks means that there are no significant differences when compared with values of Me₂SO at the corresponding time points. B. in HacaT cells. Statistically significant differences were determined by Dunnett's multiple comparisons test and are represented in asterisks: *p < 0.05, and **p < 0.005, two-way ANOVA. The absence of asterisks means that there are no significant differences when compared with values of Me₂SO at the corresponding time points.